molekulární genetika

kódující&= #13;

paměťové = ;

pozitivní

+ DNA vlákno=

= 5´

= ATG

= GAA

= GAG

= CTC

= ACG

= GCT

3´<= /div>

= 3´

= TAC

= CTT

= CTC

= GAG

= TGC

= CGA

= 5´

antikódujíc&iacu= te;

pracovní

negativní

- DNA vlákno=

------=_NextPart_01CA7617.10E30800 Content-Location: file:///C:/62A43E09/Mol.gen.I_soubory/slide0002_image002.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhcwFBAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAAB AAEAgAAAAAECAwICRAEAOw== ------=_NextPart_01CA7617.10E30800 Content-Location: file:///C:/62A43E09/Mol.gen.I_soubory/slide0003.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" molekulární genetika

= 5´

= AUG

= GAA

= GAG

= CUC

= ACG

= GCU

3´<= /div>

mRNA

antikodony

tRNA

= 3´

= UAC

5´ =

= 3´

= CUU

5´…..

polypeptid

Met – Glu R= 11; Glu – Leu- Thr- Ala

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= Je dán úsek paměťového vlákna DNA genu = pro lidský inzulín:

= 5´

= ATG

= GCC

= CTG

= TGG

= ATG

= CGC

3´<= /div>

Odvoďte a zapište:

a) komplementární pracovní vlákno DNA

b) mRNA

c) kódovaný polypeptid

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Řešen&iacu= te;:

= 3´

= TAC

= CGG

= GAC

= ACC

= TAC

= GCG

5´<= /div>

a)

= 5´

= AUG

= GCC

= CUG

= UGG

= AUG

= CGC

3´<= /div>

b)

c)

Met – Ala R= 11; Leu – Trp – Met - Arg

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a)

=

31

A A

       AUG=

b)

31

U U

        AUG

met 9lys asn glu 9lys

(met 9phe tyr val 8phe leu cys= 9phe) ¥

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c)

31

C C

        AUG

d)

31

G G

        AUG

(met 9pro his ala 9pro cys 9 p= ro) ¥

(met 9gly asp 10gly trp 9gly) = ¥

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<= u>RESTRIKČNÍ ENDONUKLEÁZY

ECo RI – Escherichia coli – restriktáza I

Taq I – 5´T C G A3´ … 5&a= cute;A C G = G T C G A A T T3´ <= /div>
Taq I –      5´T C G A3´ … 5&a= cute;A C G = G T C G A A T T3´=

3´= A G C = T5´ … 3´T G C C A G C T T A A5´ <= /div>
Hae II –      5´G G   C C 3´

C C   G G

Bam HI – G | G A T= C C

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Ide= ntifikace restrikčních míst

&Uacut= e;kol č. 1, str. 71 <= /div>

restrikč= ní místo

Alu I

AG / CT

Sau 3A

/ GTAC

Msp I

C / CGC

5´G.G.G.C.G.T.A.C.A.T.A.G.C= .T.A.A.T.G.G.C.A.A.G.C.T.A.T.G.G.T.3´ <= /div>

Najd&#= 283;te restrikční místa pro nabídnuté enzymy na uvedeném vlákně:=

=

=

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file:///C:/62A43E09/Mol.gen.I_soubory/slide0010_image027.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhEAA7AHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAMAAgAK ADYAggAAADIyADMzADQ0ADU1ADc3AEREAAECAwNWCBoi+qo5GNugQOJssfYd9YkhNJrlc6ppNZHC BW+SjL63zeK77tItDs/lEwJjoKGwuCImj8wG9JmMKmtUmHX6IBi9BYlYKhy7zIAw+jwUJ4fqTUa+ SQAAOw== ------=_NextPart_01CA7617.10E30800 Content-Location: file:///C:/62A43E09/Mol.gen.I_soubory/slide0011.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" molekulární genetika

Ide= ntifikace restrikčních míst

&Uacut= e;kol č. 1, str. 71 <= /div>

C / CGC

Msp I

/ GTAC

Sau 3A

AG / CT

Alu I

restrikč= ní místo

5´G.G.G.C.G.T.A.C.A.T.A.G.C= .T.A.A.T.G.G.C.A.A.G.C.T.A.T.G.G.T.3´ <= /div>

Najd&#= 283;te restrikční místa pro nabídnuté enzymy na uvedeném vlákně:=

2x=

1x

0 <= /div>

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Ide= ntifikace restrikčních míst

&Uacut= e;kol č. 2, str. 71 <= /div>

Kterou= z nabízených restriktáz můžete použ&iacu= te;t :

a) pro linearizaci dané cirkulární struktury =

b) pro vyštěpení takového úseku, aby obsahoval pol= y-A sekvenci.

G / GATCC

Bam HI

CGAT / CG

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ACIAggAAADMzADU1ADQ0ADc3ADIyAEREAAECAwPrCLrc/hAOMCu9M+rNu28BEAZkaZ5oWors576Q Ks80Dd9arbMjr8sXnGv0I7WEjEmhGBiEkA4iMCSAwnq2qnWWsXoZNtz0S4ZUgR9Vee1JddxPtjwn QkXc83zHeXr09YBpJV0KfEaBiG+DC3+JjjklBAomj5WQhyuWmn5GApmboJOYh6GgmZSlmoaiJJKp jyZdqK+Js6yktHONjLu5Zb1glK6+UGe2nMDEgnYchrHKy8zRydB1eDfGcLkhE2NeP5rgclLg3QBV WhvoPNbg6YFLTPLzx5ZV3fT5KxPv0Pj6dghVe7Gu4LmD/RIlAAA7 ------=_NextPart_01CA7617.10E30800 Content-Location: file:///C:/62A43E09/Mol.gen.I_soubory/slide0013.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" molekulární genetika

Ide= ntifikace restrikčních míst

&Uacut= e;kol č. 2, str. 71 <= /div>

Kterou= z nabízených restriktáz můžete použ&iacu= te;t :

a) pro linearizaci dané cirkulární struktury =

b) pro vyštěpení takového úseku, aby obsahoval pol= y-A sekvenci.

G / GATCC

Bam HI

CGAT / CG

Pvu I

T / CGA

Taq I

restrikč= ní místo

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2 místa - vyštěpení

1 m&ia= cute;sto - linearizace

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Anal&y= acute;za Southernova blotu. Popis metody (schéma)

Izolac= e DNA

DNA

Kvantita

Kvalita

Š= těpení (restrikce)

Restrikční endonukleázy

=

Elektr= oforéza

Bloting

Hybrid= izace

Autora= diografie

Zna= 69;ená sonda

Ode= 69;ítání

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Analýza Southernova blotu. Popis hybridizace

DNA&= #13;

Sonda<= /b>&= #13;

Zah= 45;átí

(denat= urace)

Ochlazení = ;

(renaturace)

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Analýza Southernova blotu.
Popis metody

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&Uacut= e;kol č. 4, str. 74     Gen G =

= = = = <= ![endif]> = = = = = = = <= ![endif]> = = = = = = = = = = = =

1 kb

5´

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Taq I

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=

sonda= :

cDNA

genomová<= /b><= b>

Výsledky:

a)   na 4 nebo 5 fragmentů:

3kb, 8kb, 6kb/2kb+4kb, 7kb

b) 3 fragmenty: 3kb= , 8kb, 7kb

c) ne=

d) ano

     4kb (alela +)   6kb (alela -)

=

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p6342sO7/yAoHUZhnmiqFognBHAsz3QwuHVe392r/7DbyAfU8TjE4g6nzB0PyaZMyBkQrtisdksw hL5gkSZDHlsiADs= ------=_NextPart_01CA7617.10E30800 Content-Location: file:///C:/62A43E09/Mol.gen.I_soubory/slide0023.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" molekulární genetika

Mimoge= nová sonda

=

<= span lang=3DCS style=3D'mso-special-format:lastCR;display:none'>

E1

E1

E1

E1

E1

E1

E1

E2

E2

E2

E2

E2

E2

2 cM

M

sledovaný gen

sonda

E2

restrikční fragmenty detekované sondou:

        = ;            &n= bsp;            = ; (E1)

               = ; (E<= /span>2 )

               = ; (E<= /span>2 )= 3;

<= span lang=3DCS style=3D'mso-special-format:lastCR;display:none'>

=

E1

E1

E1

E1

E1

E1

E1

E2

E2

E2

E2

E2

2 cM

P

sledovaný gen

sonda

E2

E1 – restrikční endonukleáza 1

E2 – restrikční endonukleáza 2

M – fragment maternálního chromosomu
P – fragment paternálního chromosomu
¯<= span lang=3DCS style=3D'font-size:64%;text-shadow:auto;color:#FFFF66'> - místa štěpení restriktázami (cílové sekvence)

2cM – vzdálenost sledovaným genem a místem, kde hybridizuje sonda, resp. mís= tem restrikce

<= span lang=3DCS style=3D'mso-special-format:lastCR;display:none'>

=

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R0lGODlhCgAKAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAAJ AAkAgQAAAP9mAP9nAAECAwIQRIwnwc3m2oqPskkhxVHHAgA7 ------=_NextPart_01CA7617.10E30800 Content-Location: file:///C:/62A43E09/Mol.gen.I_soubory/slide0023_image144.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhCgAKAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAAJ AAkAgQAAAP9mAP9qAP9oAAINRI6pZ+vYnotSgXEzLgA7 ------=_NextPart_01CA7617.10E30800 Content-Location: file:///C:/62A43E09/Mol.gen.I_soubory/slide0024.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" molekulární genetika

=

<= span lang=3DCS style=3D'mso-special-format:lastCR;display:none'>

<= span lang=3DCS style=3D'mso-special-format:lastCR;display:none'>

1

Rodina je tzv. neinformativní. = = Vš= ichni jsou homozygoti v délce restrikč= ních frag= mentů. = (Rodič= e heterozygoti ve sledovaném genu). Neví= ;me = vš= ak, který fragment 1 předali, = = zda s mutovanou nebo zdravou alelou.

=

=

1

2

Rodina je opět <= /span>neinfor= mativní, protože nelze rozhodnout, se kterým fragmentem je ve vazb= ě= mutovaná alela u otce a matky.

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Z otcovy strany fragment 2 ve vazbě= s mutov= anou alelou, protože = postiž= en&yacu= te; syn má fragment 2 od otce. M= atka je totiž homozygot (1,1) a tud&iac= ute;ž= p<= /span>ř= ed&aacu= te;vá potomkům pouze = fragmen= ty 1. Nelze ale rozhodnout, zda druhému dítěti př= edala fragment se zdravou nebo mutovanou alelou. <= /b>Druh&ea= cute; dítě bude buď v 50% = postiž= eno neb= o v 50% přenašeč.

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Zde nebude analýza úspě= šná, chybí-li DNA zem= ř= el&eacu= te;ho syna. Riziko 25%. Rodiče jsou = heteroz= ygoti – měli postiž= en&eacu= te; dítě AR chorobo= u <= /span>

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Postižen&yacu= te; syn je homozygot vdélce res= trikčnch frag= mentů = (1,1). Z toho plyne, že = fragmen= ty 1 jsou u obou rodičů ve vazbě s mutovanou alelou. Jestliže druhé dítě je = homozyg= ot (2,2), znamená to, že bude zdravý homozygot ve sledova= ném genu.

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Otec (1,1), matka (1,2), postižen&yacu= te; syn (1,2) musí mít fragment 1 od otce a od matky fragment 2, který je ve vazbě= s mutovanou alelou. Druhé dítě (1,1) má od matky fragment 1, který nese zdravou alelu a od otce také fragmen= t 1, ale nevíme, zda s mutovan= ou nebo zdravou alelou. Progn&o= acute;za: 50% heterozygot, 50% zdrav&y= acute; homozygot ve sledovaném genu - fenotypově zdravé.

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ABAAgAAAAP//AAJihI+pywEeopzwsYuz3gbSD4afxZXmIqbqSp0uplrVKz8p/Yo4re+IlGg5fL7e DthpEZc/EA6oZEp/tijHOM0eQteR9ovywqzgcpO8RZvXSejZyY5XJ2msfE0eku78av+/UQAAOw== ------=_NextPart_01CA7617.10E30800 Content-Location: file:///C:/62A43E09/Mol.gen.I_soubory/slide0028.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" molekulární genetika

Jsou j= edinci II/2 a II/3 heterozygoti pro mutovanou alelu sledov= aného AR onemocnění? (vazba úplná)

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&Uacut= e;kol č. 1b, str. 81 <= /div>

1

2

I

II

= 1

= 1

= 2

=

= 2

=

= 3

Jsou jedinci II/2 a II/3 heterozygoti pro mutovanou alelu sledovaného AR onemocnění? (vazba úplná)<= /b>

II/2 AA nebo Aa

II/3 AA nebo Aa =

Vyše= tření= ; je neúspěšné (neinformativní z hlediska ur<= /b>čení= ; = heterozyg= ocie jedinců II/2 a II/3).&#= 13;

Aa=

Aa=

a<= /span>

a<= /span>

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&Uacut= e;kol č. 1c, str. 81 <= /div>

<= /div>

1

2

I

II

1

=

2

=

3

=

2

=

1

Je dcera II/3 přena#= 3;eč= kou (heterozygot) pro mutovanou alelu sledova= ného GR onemocnění? (vazba úplná)

X<= /span>+Y

X<= /span>+Xh=

X<= /span>hY

X<= /span>+Y

X<= /span>h=

X<= /span>h=

X<= /span>h=

X<= /span>+=

X<= /span>+=

X<= /span>+=

X<= /span>+=

II/3 ano<= /b> <= /div>

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file:///C:/62A43E09/Mol.gen.I_soubory/slide0031.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" molekulární genetika

1

2

I

II

1

2

=

3

2

=

1

&Uacut= e;kol č. 1d, str. 81 <= /div>

Je dcera II/3 přena#= 3;eč= kou (heterozygot) pro mutovanou alelu sledova= ného GR onemocnění? (vazba úplná)

II/3 = ne <= /div>

X<= /span>h=

X<= /span>h=

X<= /span>+=

X<= /span>+=

X<= /span>+=

X<= /span>+=

X<= /span>+=

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IsWin("PPTSld") && parent.IsFramesMode() ) padding=3D6 cltWidth=3Ddocument.body.clientWidth-padding cltHeight=3Ddocument.body.clientHeight-padding factor=3D(1.0*cltWidth)/g_origW if( cltHeight < g_origH*factor ) factor=3D(1.0*cltHeight)/g_origH newSize =3D g_origSz * factor if( newSize < 1 ) newSize=3D1 s=3DSlideObj.style s.fontSize=3DnewSize+"px" s.posWidth=3Dg_origW*factor s.posHeight=3Dg_origH*factor s.posLeft=3D(cltWidth-s.posWidth+padding)/2 s.posTop=3D(cltHeight-s.posHeight+padding)/2 if( g_scaleHyperlinks ) ScaleHyperlinks( factor ) } function _InitAnimations() { animRuntimeInstalled =3D ''+document.body.localTime !=3D 'undefined'; isFullScreen =3D (window.name =3D=3D "PPTSld") && !parent.IsFramesMode(); g_animUseRuntime =3D g_showAnimation && animRuntimeInstalled && !(isFullSc= reen && parent.IsSldVisited()); if( g_animUseRuntime ) { collSeq =3D document.all.tags("seq"); if( collSeq !=3D null ) { for(ii=3D0;ii numSlds ) gSldJumpIdx =3D numSlds; if ( gSldJumpIdx >=3D 0 ) { if ( 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g_animUseRuntime =3D false; var g_animItemsToHide, g_animInteractiveItems, g_animSlideTime; var g_animMainSequence =3D null; var ENDSHOW_MESG=3D"Konec prezentace, klepnutím ukončíte= předvádění.", SCREEN_MODE=3D"Frames", gIsEndShow= =3D0, NUM_VIS_SLDS=3D25, SCRIPT_HREF=3D"script.js", FULLSCR_HREF=3D"fullscr= een.htm"; var gCurSld =3D gPrevSld =3D 1, g_offset =3D 0, gNtsOpen =3D gHasNts =3D gO= tlTxtExp =3D 0, gHasNarration =3D 0, gOtlOpen =3D true window.gPPTHTML=3DSupportsPPTHTML() var gMainDoc=3Dnew Array(new hrefList("slide0001.htm",1,-1,1),new hrefList(= "slide0002.htm",1,-1,1),new hrefList("slide0003.htm",1,-1,1),new hrefList("= slide0004.htm",1,-1,1),new hrefList("slide0005.htm",1,-1,1),new hrefList("s= lide0006.htm",1,-1,1),new hrefList("slide0007.htm",1,-1,1),new hrefList("sl= ide0009.htm",1,-1,1),new hrefList("slide0010.htm",1,-1,1),new hrefList("sli= de0011.htm",1,-1,1),new hrefList("slide0012.htm",1,-1,1),new hrefList("slid= e0013.htm",1,-1,1),new 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') doc.close() } function SetSldVisited(){ GetSldList().mList[gCurSld-1].mVisited=3Dtrue } function IsSldVisited(){ return GetSldList().mList[gCurSld-1].mVisited } function hrefList( sldHref, visible, advDelay, advClk ) { this.mSldHref=3D this.mNtsHref =3D sldHref this.mOrigVis=3D this.mVis =3D visible this.mVisited=3D false this.mAdvDelay=3D advDelay this.mAdvOnClk=3D advClk } function SldList(arr,curSld,fEnd) { this.mCurSld =3D curSld; this.mList =3D new Array(); var idx =3D 1; for(ii=3D0;ii 0) { PushSldList(sldList,fEnd); gCurSld =3D 1; } else if( PPTSld.event ) PPTSld.event.cancelBubble=3Dtrue } function PushSldList(arr,fEnd) { var ii =3D gSldStack.length; gSldStack[ii] =3D new SldList(arr,gCurSld,fEnd); GoToSld( gSldStack[ii].mList[0].mSldHref ); } function PopSldList() { if (gSldStack[gSldStack.length-1].fEndShow) EndShow() else { gCurSld =3D gSldStack[gSldStack.length-1].mCurSld; gSldStack[gSldStack.length-1] =3D null; gSldStack.length--; var sldList =3D gSldStack[gSldStack.length-1]; GoToSld( sldList.mList[gCurSld - 1].mSldHref ); } } var custShowList=3Dnew Array(); function ImgBtn( oId,bId,w,action ) { var t=3Dthis t.Perform =3D _IBP t.SetActive =3D _IBSetA t.SetInactive=3D _IBSetI t.SetPressed =3D _IBSetP t.SetDisabled=3D _IBSetD t.Enabled =3D _IBSetE t.ChangeIcon =3D null t.UserAction =3D action t.ChgState =3D _IBUI t.mObjId =3D oId t.mBorderId=3D bId t.mWidth =3D w t.mIsOn =3D t.mCurState =3D 0 } function _IBSetA() { if( this.mIsOn ) { obj=3Dthis.ChgState( gHiliteClr,gShadowClr,2 ) obj.style.posTop=3D0 } } function _IBSetI() { if( this.mIsOn ) { obj=3Dthis.ChgState( gFaceClr,gFaceClr,1 ) obj.style.posTop=3D0 } } function _IBSetP() { if( this.mIsOn ) { obj=3Dthis.ChgState( gShadowClr,gHiliteClr,2 ) obj.style.posLeft+=3D1; obj.style.posTop+=3D1 } } function _IBSetD() { obj=3Dthis.ChgState( gFaceClr,gFaceClr,0 ) obj.style.posTop=3D0 } function _IBSetE( state ) { var t=3Dthis GetObj( t.mBorderId ).style.visibility=3D"visible" if( state !=3D t.mIsOn ) { t.mIsOn=3Dstate if( state ) t.SetInactive() else t.SetDisabled() } } function _IBP() { var t=3Dthis if( t.mIsOn ) { if( t.UserAction !=3D null ) t.UserAction() if( t.ChangeIcon ) { obj=3DGetObj(t.mObjId) if( t.ChangeIcon() ) obj.style.posLeft=3Dobj.style.posLeft+(t.mCurState-4)*t.mWidth else obj.style.posLeft=3Dobj.style.posLeft+(t.mCurState-0)*t.mWidth } t.SetActive() } } function _IBUI( clr1,clr2,nextState ) { var t=3Dthis SetBorder( GetObj( t.mBorderId ),clr1,clr2 ) obj=3DGetObj( t.mObjId ) obj.style.posLeft=3Dobj.style.posLeft+(t.mCurState-nextState)*t.mWidth-obj= .style.posTop t.mCurState=3DnextState return obj } function TxtBtn( oId,oeId,action,chkState ) { var t=3Dthis t.Perform =3D _TBP t.SetActive =3D _TBSetA t.SetInactive=3D _TBSetI t.SetPressed =3D _TBSetP t.SetDisabled=3D _TBSetD t.SetEnabled =3D _TBSetE t.GetState =3D chkState t.UserAction =3D action t.ChgState =3D _TBUI t.mObjId =3D oId t.m_elementsId=3D oeId t.mIsOn =3D 1 } function _TBSetA() { var t=3Dthis if( t.mIsOn && !t.GetState() ) t.ChgState( gHiliteClr,gShadowClr,0,0 ) } function _TBSetI() { var t=3Dthis if( t.mIsOn && !t.GetState() ) t.ChgState( gFaceClr,gFaceClr,0,0 ) } function _TBSetP() { if( this.mIsOn ) this.ChgState( gShadowClr,gHiliteClr,1,1 ) } function _TBSetD() { this.ChgState( gFaceClr,gFaceClr,0,0 ) this.mIsOn =3D 0 } function _TBSetE() { var t=3Dthis if( !t.GetState() ) t.ChgState( gFaceClr,gFaceClr,0,0 ) else t.ChgState( gShadowClr,gHiliteClr,1,1 ) t.mIsOn =3D 1 } function _TBP() { var t=3Dthis if( t.mIsOn ) { if( t.UserAction !=3D null ) t.UserAction() if( !t.GetState ) return if( t.GetState() ) t.SetPressed() else t.SetActive() } } function _TBUI( clr1,clr2,lOffset,tOffset ) { SetBorder( GetObj( this.mObjId ),clr1,clr2 ) Offset( GetObj( this.m_elementsId ),lOffset,tOffset ) } function Offset( obj, top, left ){ obj.style.top=3Dtop; obj.style.left=3Dle= ft } function SetBorder( obj, upperLeft, lowerRight ) { s=3Dobj.style; s.borderStyle =3D "solid" s.borderWidth =3D 1 s.borderLeftColor =3D s.borderTopColor =3D upperLeft s.borderBottomColor=3D s.borderRightColor =3D lowerRight } function GetBtnObj(){ return gBtnArr[window.event.srcElement.id] } function BtnOnOver(){ b=3DGetBtnObj(); if( b !=3D null ) b.SetActive() } function BtnOnDown(){ b=3DGetBtnObj(); if( b !=3D null ) b.SetPressed() } function BtnOnOut(){ b=3DGetBtnObj(); if( b !=3D null ) b.SetInactive() } function BtnOnUp() { b=3DGetBtnObj() if( b !=3D null ) b.Perform() else Upd() } function GetNtsState(){ return parent.gNtsOpen } function GetOtlState(){ return parent.gOtlOpen } function GetOtlTxtState(){ return parent.gOtlTxtExp } function NtsBtnSetFlag( fVal ) { s=3Ddocument.all.item( this.m_flagId ).style s.display=3D"none" if( fVal ) s.display=3D"" else s.display=3D"none" } function _BSetA_Border(){ b =3D gBtnArr[this.mObjId]; if( b !=3D null ) b.S= etActive() } function _BSetI_Border(){ b =3D gBtnArr[this.mObjId]; if( b !=3D null ) b.S= etInactive() } function _BSetP_Border(){ b =3D gBtnArr[this.mObjId]; if( b !=3D null ) b.S= etPressed() } function _BSetA_BorderImg() { b =3D gBtnArr[this.mBorderId] if( b !=3D null && this.mIsOn && !b.GetState() ) { obj=3Dthis.ChgState( gHiliteClr,gShadowClr,2 ) obj.style.posTop=3D0 } } function _BSetI_BorderImg() { b =3D gBtnArr[this.mBorderId] if( b !=3D null && this.mIsOn && !b.GetState() ) { obj=3Dthis.ChgState( gFaceClr,gFaceClr,1 ) obj.style.posTop=3D0 } } var gHiliteClr=3D"THREEDHIGHLIGHT",gShadowClr=3D"THREEDSHADOW",gFaceClr=3D"= THREEDFACE" var gBtnArr =3D new Array() gBtnArr["nb_otl"] =3D new TxtBtn( "nb_otl","nb_otlElem",parent.ToggleOtlPan= e,GetOtlState ) gBtnArr["nb_otlElem"] =3D new TxtBtn( "nb_otl","nb_otlElem",parent.ToggleOt= lPane,GetOtlState ) gBtnArr["nb_nts"] =3D new ImgBtn( "nb_nts","nb_ntsBorder",10,parent.ToggleN= tsPane ) gBtnArr["nb_nts"].SetActive =3D _BSetA_BorderImg; gBtnArr["nb_nts"].SetInactive =3D _BSetI_BorderImg; gBtnArr["nb_ntsBorder"] =3D new TxtBtn( "nb_ntsBorder","nb_ntsElem",parent.= ToggleNtsPane,GetNtsState ) gBtnArr["nb_ntsElem"] =3D new TxtBtn( "nb_ntsBorder","nb_ntsElem",parent.To= ggleNtsPane,GetNtsState ) gBtnArr["nb_prevBorder"] =3D gBtnArr["nb_prev"]=3D new ImgBtn( "nb_prev","n= b_prevBorder",30,parent.GoToPrevSld ) gBtnArr["nb_nextBorder"] =3D gBtnArr["nb_next"]=3D new ImgBtn( "nb_next","n= b_nextBorder",30,parent.GoToNextSld ) gBtnArr["nb_sldshw"]=3D new ImgBtn( "nb_sldshw","nb_sldshwBorder",18,parent= .FullScreen ) gBtnArr["nb_sldshwBorder"] =3D new TxtBtn( "nb_sldshw","nb_sldshwBorder",pa= rent.FullScreen,null ) gBtnArr["nb_sldshwBorder"].SetActive =3D _BSetA_Border; gBtnArr["nb_sldshwBorder"].SetInactive =3D _BSetI_Border; gBtnArr["nb_sldshwText"] =3D new TxtBtn( "nb_sldshw","nb_sldshwText",parent= .FullScreen,null ) gBtnArr["nb_sldshwText"].SetActive =3D _BSetA_Border; gBtnArr["nb_sldshwText"].SetInactive =3D _BSetI_Border; gBtnArr["nb_voice"] =3D gBtnArr["nb_voiceBorder"] =3D new ImgBtn( "nb_voice= ","nb_voiceBorder",18,parent.ToggleVNarration ) gBtnArr["nb_otlTxtBorder"] =3D gBtnArr["nb_otlTxt"]=3D new ImgBtn( "nb_otlT= xt","nb_otlTxtBorder",23,parent.ToggleOtlText ) gBtnArr["nb_ntsBorder"].m_flagId=3D "nb_nts" gBtnArr["nb_ntsBorder"].SetFlag =3D NtsBtnSetFlag gBtnArr["nb_otlTxt"].ChangeIcon=3D GetOtlTxtState var sNext=3D"Další",sPrev=3D"Předchozí",sEnd=3D"Kon= ec prezentace",sFont=3D"Arial",sArrow=3D"Šipka",sFreeform=3D"Voln&yacu= te; tvar",sRect=3D"Obdélník",sOval=3D"Ovál" function ShowMenu() { BuildMenu(); var doc=3DPPTSld.document.body,x=3DPPTSld.event.clientX+doc.scrollLeft,y= =3DPPTSld.event.clientY+doc.scrollTop m =3D PPTSld.document.all.item("ctxtmenu") m.style.pixelLeft=3Dx if( (x+m.scrollWidth > doc.clientWidth)&&(x-m.scrollWidth > 0) ) m.style.pixelLeft=3Dx-m.scrollWidth m.style.pixelTop=3Dy if( (y+m.scrollHeight > doc.clientHeight)&&(y-m.scrollHeight > 0) ) m.style.pixelTop=3Dy-m.scrollHeight m.style.display=3D"" } function _CM() { if( !parent.IsFullScrMode() ) return; if(!PPTSld.event.ctrlKey) { ShowMenu() return false } else HideMenu() } function BuildMenu() { if( PPTSld.document.all.item("ctxtmenu") ) return var mObj=3DCreateItem( PPTSld.document.body ) mObj.id=3D"ctxtmenu" mObj.style.visibility=3D"hidden" var s=3DmObj.style s.position=3D"absolute" s.cursor=3D"default" s.width=3D"120px" SetCMBorder(mObj,"menu","black") var iObj=3DCreateItem( mObj ) SetCMBorder( iObj, "threedhighlight","threedshadow" ) iObj.style.padding=3D2 CreateMenuItem( iObj,sNext,M_GoNextSld,M_True ) CreateMenuItem( iObj,sPrev,M_GoPrevSld,M_HasPrevSld ) CreateSeparator( iObj ) CreateMenuItem( iObj,sEnd,M_End,M_True ) mObj.style.visibility=3D"visible" } function Cancel() { window.event.cancelBubble=3Dtrue; window.event.returnVa= lue=3Dfalse } function Highlight() { ChangeClr("activecaption","threedhighlight") } function Deselect() { ChangeClr("threedface","menutext") } function Perform() { e=3DPPTSld.event.srcElement if( e.type=3D=3D"menuitem" && e.IsActive() ) e.Action() else PPTSld.event.cancelBubble=3Dtrue } function ChangeClr( bg,clr ) { e=3DPPTSld.event.srcElement if( e.type=3D=3D"menuitem" && e.IsActive() ) { e.style.backgroundColor=3Dbg e.style.color=3Dclr } } function M_HasPrevSld() { return( parent.HasPrevSld() ) } function M_GoNextSld() { if( gIsEndShow ) M_End(); 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charset="us-ascii" molekulární genetika ------=_NextPart_01CA7617.10E30800 Content-Location: file:///C:/62A43E09/Mol.gen.I_soubory/outline.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii"

Po= známky

Prezentac= e

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MOLEKULÁR= NÍ GENETIKA I

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= = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = =
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1	MOLEKULÁR= NÍ GENETIKA I Stomatologie
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