Molekulární genetika IV

SEKVENACE DNA

externí soubory:

= http://ww= w.dnalc.org/ddnalc/resources/animations/sangerseq.exe

http://www.dnalc.org/ddnalc/resources/ani= mations/cycseq.exe

&#= 13;

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Úkol č. 5a, str. 94

5R= 17;-GAGCGATCGCC= TCACC= GGCCCATC-3&= #8217;

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Úkol č. 5b, str. 94

5R= 17;-GAGCGATCGCC= TCACC= GGCCCATC-3&= #8217;

= 5R= 17;-GAGCGATCGCC= TCACC= GGCCCATC-3&= #8217;

<= span lang=3DCS style=3D'mso-special-format:lastCR;display:none'>

250

…. …. …. GAG CGA UCG<= /b> CCU<= /b> CAC<= /b> CGG<= /b> CCC<= /b> AUC<= /b>… …. ….

Glu Arg Ser Pro His Arg Pro Ile

250

…. …. …. GAG CGA UGG CCU<= /b> CAC<= /b> CGG<= /b> CCC<= /b> AUC<= /b>… …. ….

Glu Arg Trp Pro His Arg Pro Ile

=

Ser252Trp

S252W

8

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„Příští generace“ sekvenačních platform, př.: SOLiD

druhá baze

dekódování

Barevný prostor

sekvence v barevném prostoru

Možné dinukleotidy

Nultá baze

Dekódované

dinukleotidy

Sekvence ve

standardním formátu

9

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SOLiD: přepište výsledky z barevného prostoru do sekvence (první baze je G). Která sekvence obsahuje mutaci a která chy= bu?

Kontrolní sekvence

Pacient 1

Pacient 2

10

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Kontrolní sekvence

Pacient 1

Pacient 2

Pacient 1 – mutace C na G, pacient 2 – chyba stanovení, nutno předělat

11

SOLiD: přepište výsledky z barevného prostoru do sekvence (první baze je G). Která sekvence obsahuje mutaci a která chy= bu?

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Úkol č. 6, str. 70

1308

…. …. …. AAA GAA AAG ATT GGA ACT AGG TCA … …. ….=

Lys    Glu    Lys    Ile   Gly    Thr    Arg   Ser

1315

=

…. …. …. AAA

GAT

TGG

AAC

TAG

Lys  &= nbsp; Asp    Trp   Asn Term

A.

B.

…. …. …. GUG CAG GGT &= #8230; …. ….

Leu   Gln   Gly

1339

T

Leu   Term

C.

…. …. …. GCC AGG CAC … …. ….<= /span>

Ala   Arg    His

1349

T

Ala   Trp    His

Posun čtecího rámce<= /span>

Nonsense mutace

(terminace translace)

Missense mutace

(záměna aminokyseliny)

12

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charset="us-ascii" Molekulární genetika IV

Achondr= oplazie je nejčastější genetickou příčinou malého vzrůstu. Bylo zjištěno, že příčinou onemocnění je mutace v genu FGFR3. A= si 95% pacientů má jediný typ mutace, a sice= 1138G>= A, což = vede k záměně glycinu v pozici 380 na arginin. Touto mutac&iacut= e; se zároveň vytváří restrikční místo pro SfcI

PCR:

55bp

109bp

13

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Ú= ;kol: stanovte riziko, že v manželům, kteří trp&iacut= e; achondroplazií se narodí dítě postižen&eacu= te; homozygotní formou tohoto onemocnění (tento genotyp je neslučitelný s postnatálním životem, navíc těhotenství matek s achondroplazií pro n&#= 283; představuje značnou zátěž):

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Shiang et al., 1994

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Ře= šení: oba rodiče jsou heterozygoti pro G380R, stejně tak otec těhotné ženy. DNA plodu obsahuje pouze alelu s přítomností restrikčního místa, tedy mutovanou, plod je tudíž homozygot (AA) a je indikováno přerušení těhotenství . Pozn.: V rodině = otce se jedná o novou mutaci (rodiče zdrávi)

aa

aa

Aa

Aa

Aa

aa

AA

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------=_NextPart_01C945BF.EA83B520 Content-Location: file:///C:/4579C638/MolGenIV_v2008_soubory/slide0079.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" Molekulární genetika IV

Dom&aac= ute;cí úkol:

U mal&e= acute; části nemocných je achondroplazie způsobena jinou mutací v genu FGFR3, ve stejném kodonu, 1138G>= C. = ;

= a) K jaké změně aminokyseliny dojde?
b) Navrhněte přímou DNA diagnostiku - bližší informace na stránkách ústavu (klikni z= de)

=

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------=_NextPart_01C945BF.EA83B520 Content-Location: file:///C:/4579C638/MolGenIV_v2008_soubory/slide0062.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" Molekulární genetika IV

Reakce

enzym&= #13;

délka fragmentu (kb)

1.

Pst I&= #13;

6,4 a 3,6&= #13;

2.

Bgl II&= #13;

8,8 a 1,2 &= #13;

3.

Pst I i Bgl II&= #13;

5,2, 3,6 a 1,2&= #13;

Nakresle= te restrikční mapu t<= /b>éto 10 kb dlouhé= sekvence DNA s vyznačenými cílovými místy pro= oba uvedené enzymy.

Sekvence DNA o délce 10kb obsahuje jedno cílové místo pro restrikční endonukleázu Pst I a jedno místo pro enzym Bgl II. Byly provedeny tři samostatné reakce:

1.= restrikce pouze s enzymem Pst I

2.= restrikce pouze s enzymem Bgl II

3.= dvojitá restrikce s oběma enzymy Pst I i Bgl II najednou

&= #13;

Při po= kusech byly získány fragmenty těchto délek:<= /nobr>

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10 kb

3,6 kb

Pst I

=

6,4 kb

1,2 kb

Bgl II

=

8,8 kb

3,6 kb

Pst I + Blg II

=

5,2 kb

1,2 kb

=

3,6 kb

Pst I + Blg II

5,2 kb

1,2 kb

3,6 kb

Pst I + Blg II

5,2 kb

1,2 kb

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Úkol č. 4, str. 83

…

Lys

Ser

Phe

Cys

Asn

Leu

Ala

Ala

Lys

…

…

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%'>Lys

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%'>Val

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%'>Phe

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%'>Ala

Ile

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%'>STOP

20

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Úkol č. 4, str. 83

…

Lys

Ser

Phe

Cys

Asn

Leu

Ala

Ala

Lys

…

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%;color:blue'>AAA<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%;color:blue'>UCG<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%;color:blue'>UUU<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%;color:blue'>UGC<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

AAC<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%;color:blue'>CUG<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

GCG<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%;color:blue'>GCG<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%;color:blue'>AAA<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

AAG<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

UCA<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%;color:blue'>UUC<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%;color:blue'>UGU<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

AAU<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

CUA<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

GCA<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%;color:blue'>GCA<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

AAG<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

UCC<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

CUC<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

GCC<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

GCC<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%;color:blue'>UCU<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%;color:blue'>CUU<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

GCU<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%;color:blue'>GCU<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

AGC<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

UUG<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%;color:blue'>AGU<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

UUA<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

…

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%'>Lys

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%'>Val

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%'>Phe

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%'>Ala

Ile

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%'>STOP

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%;color:blue'>AAA<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%;color:blue'>GUG<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%;color:blue'>UUU<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%;color:blue'>GCG<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

AUA<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%;color:blue'>UAG<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

AAG<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

GUA<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

UUC<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

GCA<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

AUC<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

UAA<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%;color:blue'>GUC<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%;color:blue'>GCC<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

AUU<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%;color:blue'>UGA<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

GUU<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

GCU<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

21

------=_NextPart_01C945BF.EA83B520 Content-Location: file:///C:/4579C638/MolGenIV_v2008_soubory/slide0069.xml Content-Transfer-Encoding: quoted-printable Content-Type: text/xml; charset="utf-8" ------=_NextPart_01C945BF.EA83B520 Content-Location: file:///C:/4579C638/MolGenIV_v2008_soubory/slide0069_image151.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlh0AEvAXcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAAB AAEAgAAAAAECAwICRAEAOw== ------=_NextPart_01C945BF.EA83B520 Content-Location: file:///C:/4579C638/MolGenIV_v2008_soubory/slide0070.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" Molekulární genetika IV

Úkol č. 4, str. 83

…

Lys

Ser

Phe

Cys

Asn

Leu

Ala

Ala

Lys

…

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%;color:blue'>AAA<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%;color:blue'>UCG<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%;color:red'>UUU=

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%;color:red'>UGC=

AAC<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%;color:blue'>CUG<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

GCG

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%;color:blue'>GCG<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%;color:blue'>AAA<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

AAG<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

UCA<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%;color:blue'>UUC<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%;color:blue'>UGU<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

AAU=

C= UA=

GCA

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%;color:blue'>GCA<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

AAG<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

UCC<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

CUC<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

GCC

GCC<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%;color:blue'>UCU<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%;color:blue'>CUU<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

GCU

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%;color:blue'>GCU<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

AGC<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

UUG<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%;color:blue'>AGU=

U= UA=

…

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%'>Lys

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%'>Val

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%'>Phe

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%'>Ala

Ile

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%'>STOP

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%;color:blue'>AAA<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%;color:blue;mso-color-in= dex: 0'>GUG

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%;color:red'>UUU=

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%;color:blue'>GCG<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

AUA<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%;color:red'>UAG=

AAG<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

GUA<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

UUC<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

GCA=

AUC=

UAA<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%;color:blue'>GUC<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%;color:blue'>GCC<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

AUU=

<= span style=3D'mso-bidi-font-family:Arial;font-size:250%;color:blue'>UGA<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

GUU=

GCU<= span style=3D'font-size:250%;color:yellow;mso-color-index:1;mso-special-format:= lastCR; display:none'>

Řešení: delece A v poz= ici 4 (pokud je první kodon AAG, nebo zkrácení A4

na A3, pokud je první kodon AAA)

22

------=_NextPart_01C945BF.EA83B520 Content-Location: file:///C:/4579C638/MolGenIV_v2008_soubory/slide0070.xml Content-Transfer-Encoding: quoted-printable Content-Type: text/xml; charset="utf-8" ------=_NextPart_01C945BF.EA83B520 Content-Location: file:///C:/4579C638/MolGenIV_v2008_soubory/slide0070_image152.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlh0AEvAXcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAAB AAEAgAAAAAECAwICRAEAOw== ------=_NextPart_01C945BF.EA83B520 Content-Location: file:///C:/4579C638/MolGenIV_v2008_soubory/slide0080.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" Molekulární genetika IV

¨Kt= erý ze tří podezřelých je nejpravděpodobněji vinen?

Autorad= iogram Southernova blotu, hybridizovaného se sondou proti hojné repetitivní sekvenci (typu minisatelitu). Alely různé délky z mnoha lokusů se skládají dohromady v „otisk“ charakteristický pro jedince. Tato konktétní metoda již nicméně vyšla z módy.

23

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hTEerz4qr1rITyilahafbvrG8XBso72jnPom4UQihqpoCFlicD6StJ5Xi0jPyFWMOhbrxGVf9zGH rcjaiyaZxNoiwsUf/HVjLQLoaAkV5qiFLpEniryUxjBPSNarxgAphxTVZqUe9Wwe6XWNZWbguWUX hYUKVgDINF3deKkQ7YHejiboxWYkDq6tt7SnlQyU1OYb56zoTjlPIjYosD7rsKnKQCXtmdVtq/Wk QaKO5YGl4Ilcuc6JamSqQnoQyMClDTXHmZrrLMqlXybK3pT5GIzu5cmVHMoFF8PSFJmRj4pF7bHV hhiKKuk2kkyNZzO97ojRmcHmFwGOmjj939hSLI9+FdAsYJl+7pi6ba6Oo9oK2mpBz7imhuO654i5 5wDe7Aup5PJWZ0Xy2PVGqUXmDStu3B367PwFbeICZV+pIq7KImyFb+fy5A757ripZFopZvJFG0DS p/Di4TgqKPHuL//2b6EBmv/S0FrqVtX2J6PGLAJ7bl7SHy1yra6m6+a6KwNPcOZSsAJbcAMvcLvS XwT7bCsEBAA7 ------=_NextPart_01C945BF.EA83B520 Content-Location: file:///C:/4579C638/MolGenIV_v2008_soubory/slide0080_image155.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhaAEuAHcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAABn AS0AgAAAAP8AAAL/hH8ByusNn4x02oqvznz7Dn5iSI5miZ5qmiKuG8TyTNf2jef6zvf+DwwKh8Si 8YgsvhLJpvMJjUqn1KoVt1xct9yu9wsOO2HisvmMTquBiFr2DY/L5/S6/Y7P6/f8vv8PuOdmQBNo eIiYqLjI2OioWFj4OElZaXmJmdknOaPp+QkaKjoqF9lJipqqusoqKEMY0yo7S1sbavpqq7vL27uZ m+srPEwsjBtbnKy8nHoazAwdLe14rDB9jZ3N54ys7f0NTvYqGV5uPs1tfb7OTlzdDh+vmy5fb6/6 fq+/r0nP/w+QWidyAQsa/OPvoMKFd/IxfAgxS8KIFCM6rIhx4cSMdxwDXuwIct/GkCTlfSyJkt3I lCzDnWwJU9vKmDSlvayJc9nMnDyH3ewJlFe6bkGL7uJE0KjSVuRsrHjKAqrUqFSnWq2K9arWrFxX nGKyJqzYsWTLInlhNq3atWzDLmkLN67cuUfmdL27NS/evXr78v3rN3CDFwUAADs= ------=_NextPart_01C945BF.EA83B520 Content-Location: file:///C:/4579C638/MolGenIV_v2008_soubory/slide0071.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" Molekulární genetika IV

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

Val

His

Leu

Thr

Pro

Glu

Glu

Lys

Ser

Ala

Val

Thr

Ala

Leu

Trp

GAG

GAG

AAG

U

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

16

17

18

19

20

21

22

23

24

25

26

27

28

29

30

Gly

Lys

Val

Asn

Val

Asp

Glu

Val

Gly

Gly

Glu

Ala

Leu

Gly

Arg

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

31

32

33

34

35

36

37

38

39

40

41

42

43

44

45

Leu

Leu

Val

Val

Tyr

Pro

Trp

Thr

Gln

Arg

Phe

Phe

Glu

Ser

Phe

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

Úkol č. 3, str. 68

24

------=_NextPart_01C945BF.EA83B520 Content-Location: file:///C:/4579C638/MolGenIV_v2008_soubory/slide0071.xml Content-Transfer-Encoding: quoted-printable Content-Type: text/xml; charset="utf-8" ------=_NextPart_01C945BF.EA83B520 Content-Location: file:///C:/4579C638/MolGenIV_v2008_soubory/slide0071_image156.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhoQFYAXcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAAB AAEAgAAAAAECAwICRAEAOw== ------=_NextPart_01C945BF.EA83B520 Content-Location: file:///C:/4579C638/MolGenIV_v2008_soubory/slide0072.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" Molekulární genetika IV

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

Val

His

Leu

Thr

Pro

Glu

Glu

Lys

Ser

Ala

Val

Thr

Ala

Leu

Trp

GUG

CAU

CUG

ACG

CCG

GAG

GAG

AAG

UCG

GCG

GUG

ACG

GCG

CUG

UGG

GUA

CAC

CUA

ACA

CCA

= 3;

= 3;

= 3;

UCA

GCA

GUA

ACA

GCA

CUA

= 3;

GUC

= 3;

CUC

ACC

CCC

= 3;

= 3;

= 3;

UCC

GCC

GUC

ACC

GCC

CUC

= 3;

GUU

= 3;

CUU

ACU

CCU

= 3;

= 3;

= 3;

UCU

GCU

GUU

ACU

GCU

CUU

= 3;

= 3;

= 3;

UUG

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

UUG

= 3;

= 3;

= 3;

UUA

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

UUA

= 3;

16

17

18

19

20

21

22

23

24

25

26

27

28

29

30

Gly

Lys

Val

Asn

Val

Asp

Glu

Val

Gly

Gly

Glu

Ala

Leu

Gly

Arg

GGG

AAA

GUG

AAC

GUG

GAC

GAG

GUG

GGG

GGG

GAG

GCG

CUG

GGG

CGG

GGA

AAG

GUA

AAU

GUA

GAU

GAA

GUA

GGA

GGA

GAA

GCA

CUA

GGA

CGA

GGC

= 3;

GUC

= 3;

GUC

= 3;

= 3;

GUC

GGC

GGC

= 3;

GCC

CUC

GGC

CGC

GGU

= 3;

GUU

= 3;

GUU

= 3;

= 3;

GUU

GGU

GGU

= 3;

GCU

CUU

GGU

CGU

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

UUG

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

UUA

= 3;

= 3;

31

32

33

34

35

36

37

38

39

40

41

42

43

44

45

Leu

Leu

Val

Val

Tyr

Pro

Trp

Thr

Gln

Arg

Phe

Phe

Glu

Ser

Phe

CUG

CUG

GUG

GUG

UAC

CCG

UGG

ACG

CAG

CGG

UUU

UUU

GAG

UCG

UUU

CUA

CUA

GUA

GUA

UAU

CCA

= 3;

ACA

CAA

CGA

UUC

UUC

GAA

UCA

UUC

CUC

CUC

GUC

GUC

= 3;

CCC

= 3;

ACC

= 3;

CGC

= 3;

= 3;

= 3;

UCC

= 3;

CUU

CUU

GUU

GUU

= 3;

CCU

= 3;

ACU

= 3;

CGU

= 3;

= 3;

= 3;

UCU

= 3;

UUG

UUG

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

AGC

= 3;

UUA

UUA

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

AGU

= 3;

Úkol č. 3, str. 68

25

------=_NextPart_01C945BF.EA83B520 Content-Location: file:///C:/4579C638/MolGenIV_v2008_soubory/slide0072.xml Content-Transfer-Encoding: quoted-printable Content-Type: text/xml; charset="utf-8" ------=_NextPart_01C945BF.EA83B520 Content-Location: file:///C:/4579C638/MolGenIV_v2008_soubory/slide0072_image157.gif Content-Transfer-Encoding: base64 Content-Type: image/gif R0lGODlhoQFYAXcAMSH+GlNvZnR3YXJlOiBNaWNyb3NvZnQgT2ZmaWNlACH5BAEAAAAALAAAAAAB AAEAgAAAAAECAwICRAEAOw== ------=_NextPart_01C945BF.EA83B520 Content-Location: file:///C:/4579C638/MolGenIV_v2008_soubory/slide0073.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" Molekulární genetika IV

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

Val

His

Leu

Thr

Pro

Gly

Arg

Ser

xxx

xxx

xxx

xxx

xxx

xxx

Gly

GUG

CAU

CUG

ACG

CCG

G-G

GAG

AAG

UCG

GCG

GUG

ACG

GCG

CUG

UGG

GUA

CAC

CUA

ACA

CCA

= 3;

= 3;

= 3;

UCA

GCA

GUA

ACA

GCA

CUA

= 3;

GUC

= 3;

CUC

ACC

CCC

= 3;

= 3;

= 3;

UCC

GCC

GUC

ACC

GCC

CUC

= 3;

GUU

= 3;

CUU

ACU

CCU

= 3;

= 3;

= 3;

UCU

GCU

GUU

ACU

GCU

CUU

= 3;

= 3;

= 3;

UUG

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

UUG

= 3;

= 3;

= 3;

UUA

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

UUA

= 3;

16

17

18

19

20

21

22

23

24

25

26

27

28

29

30

xxx

xxx

xxx

Asn

Val

Asp

Glu

Val

Gly

Gly

Glu

Ala

Leu

Gly

Arg

GGG

AAA

GUG

AAC

GUG

GAC

GAG

GUG

GGG

GGG

GAG

GCG

CUG

GGG

CGG

GGA

AAG

GUA

AAU

GUA

GAU

GAA

GUA

GGA

GGA

GAA

GCA

CUA

GGA

CGA

GGC

= 3;

GUC

= 3;

GUC

= 3;

= 3;

GUC

GGC

GGC

= 3;

GCC

CUC

GGC

CGC

GGU

= 3;

GUU

= 3;

GUU

= 3;

= 3;

GUU

GGU

GGU

= 3;

GCU

CUU

GGU

CGU

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

UUG

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

UUA

= 3;

= 3;

31

32

33

34

35

36

37

38

39

40

41

42

43

44

45

Leu

Leu

Val

Val

Tyr

Pro

Trp

Thr

Gln

Arg

Phe

Phe

Glu

Ser

Phe

CUG

CUG

GUG

GUG

UAC

CCG

UGG

ACG

CAG

CGG

UUU

UUU

GAG

UCG

UUU

CUA

CUA

GUA

GUA

UAU

CCA

= 3;

ACA

CAA

CGA

UUC

UUC

GAA

UCA

UUC

CUC

CUC

GUC

GUC

= 3;

CCC

= 3;

ACC

= 3;

CGC

= 3;

= 3;

= 3;

UCC

= 3;

CUU

CUU

GUU

GUU

= 3;

CCU

= 3;

ACU

= 3;

CGU

= 3;

= 3;

= 3;

UCU

= 3;

UUG

UUG

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

AGC

= 3;

UUA

UUA

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

AGU

= 3;

Úkol č. 3, str. 68 a

=

=

=

<= span lang=3DCS style=3D'mso-special-format:lastCR;display:none'>

=

26

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1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

Val

His

Leu

Thr

Pro

Glu

Glu

Val

xxx

xxx

xxx

xxx

xxx

xxx

Gly

GUG

CAU

CUG

ACG

CCG

GAG

GAG

--G

UCG

GCG

GUG

ACG

GCG

CUG

UGG

GUA

CAC

CUA

ACA

CCA

= 3;

= 3;

= 3;

UCA

GCA

GUA

ACA

GCA

CUA

= 3;

GUC

= 3;

CUC

ACC

CCC

= 3;

= 3;

= 3;

UCC

GCC

GUC

ACC

GCC

CUC

= 3;

GUU

= 3;

CUU

ACU

CCU

= 3;

= 3;

= 3;

UCU

GCU

GUU

ACU

GCU

CUU

= 3;

= 3;

= 3;

UUG

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

UUG

= 3;

= 3;

= 3;

UUA

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

UUA

= 3;

16

17

18

19

20

21

22

23

24

25

26

27

28

29

30

xxx

xxx

xxx

xxx

xxx

xxx

Glu

Val

Gly

Gly

Glu

Ala

Leu

Gly

Arg

GGG

AAA

GUG

AAC

GUG

GAC

GAG

GUG

GGG

GGG

GAG

GCG

CUG

GGG

CGG

GGA

AAG

GUA

AAU

GUA

GAU

GAA

GUA

GGA

GGA

GAA

GCA

CUA

GGA

CGA

GGC

= 3;

GUC

= 3;

GUC

= 3;

= 3;

GUC

GGC

GGC

= 3;

GCC

CUC

GGC

CGC

GGU

= 3;

GUU

= 3;

GUU

= 3;

= 3;

GUU

GGU

GGU

= 3;

GCU

CUU

GGU

CGU

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

UUG

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

UUA

= 3;

= 3;

31

32

33

34

35

36

37

38

39

40

41

42

43

44

45

Leu

Leu

Val

Val

Tyr

Pro

Trp

Thr

Gln

Arg

Phe

Phe

Glu

Ser

Phe

CUG

CUG

GUG

GUG

UAC

CCG

UGG

ACG

CAG

CGG

UUU

UUU

GAG

UCG

UUU

CUA

CUA

GUA

GUA

UAU

CCA

= 3;

ACA

CAA

CGA

UUC

UUC

GAA

UCA

UUC

CUC

CUC

GUC

GUC

= 3;

CCC

= 3;

ACC

= 3;

CGC

= 3;

= 3;

= 3;

UCC

= 3;

CUU

CUU

GUU

GUU

= 3;

CCU

= 3;

ACU

= 3;

CGU

= 3;

= 3;

= 3;

UCU

= 3;

UUG

UUG

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

AGC

= 3;

UUA

UUA

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

AGU

= 3;

Úkol č. 3, str. 68 b&nb= sp;

=

=

=

=

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27

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1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

Val

His

Leu

Thr

Pro

Glu

Glu

Lys

Val

xxx

xxx

xxx

xxx

xxx

xxx

GUG

CAU

CUG

ACG

CCG

GAG

GAG

AAG

UCG

GCG

GUG

ACG

GCG

CUG

UGG

GUA

CAC

CUA

ACA

CCA

= 3;

= 3;

= 3;

UCA

GCA

GUA

ACA

GCA

CUA

= 3;

GUC

= 3;

CUC

ACC

CCC

= 3;

= 3;

= 3;

UCC

GCC

GUC

ACC

GCC

CUC

= 3;

GUU

= 3;

CUU

ACU

CCU

= 3;

= 3;

= 3;

UCU

GCU

GUU

ACU

GCU

CUU

= 3;

= 3;

= 3;

UUG

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

UUG

= 3;

= 3;

= 3;

UUA

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

UUA

= 3;

16

17

18

19

20

21

22

23

24

25

26

27

28

29

30

Gly

xxx

xxx

xxx

xxx

xxx

xxx

Val

Gly

Gly

Glu

Ala

Leu

Gly

Arg

GGG

AAA

GUG

AAC

GUG

GAC

GAG

GUG

GGG

GGG

GAG

GCG

CUG

GGG

CGG

GGA

AAG

GUA

AAU

GUA

GAU

GAA

GUA

GGA

GGA

GAA

GCA

CUA

GGA

CGA

GGC

= 3;

GUC

= 3;

GUC

= 3;

= 3;

GUC

GGC

GGC

= 3;

GCC

CUC

GGC

CGC

GGU

= 3;

GUU

= 3;

GUU

= 3;

= 3;

GUU

GGU

GGU

= 3;

GCU

CUU

GGU

CGU

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

UUG

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

UUA

= 3;

= 3;

31

32

33

34

35

36

37

38

39

40

41

42

43

44

45

Leu

Leu

Val

Val

Tyr

Pro

Trp

Thr

Gln

Arg

Phe

Phe

Glu

Ser

Phe

CUG

CUG

GUG

GUG

UAC

CCG

UGG

ACG

CAG

CGG

UUU

UUU

GAG

UCG

UUU

CUA

CUA

GUA

GUA

UAU

CCA

= 3;

ACA

CAA

CGA

UUC

UUC

GAA

UCA

UUC

CUC

CUC

GUC

GUC

= 3;

CCC

= 3;

ACC

= 3;

CGC

= 3;

= 3;

= 3;

UCC

= 3;

CUU

CUU

GUU

GUU

= 3;

CCU

= 3;

ACU

= 3;

CGU

= 3;

= 3;

= 3;

UCU

= 3;

UUG

UUG

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

AGC

= 3;

UUA

UUA

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

= 3;

AGU

= 3;

Úkol č. 3, str. 68 c&nb= sp;

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=

=

=

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FULLSCR_HREF = : FULLSCR_HREF+"#"+GetHrefObj(gCurSld).mSldHref; if(PPTNav.event.ctrlKey) { var w =3D (window.screen.availWidth * 1.0) / 2.0 var h =3D w * (PPTSld.g_origH * 1.0) / PPTSld.g_origW win =3D window.open( MHTMLPrefix+href,null,"toolbar=3D0,resizable=3D1,top= =3D0,left=3D0," + "width=3D"+ w + ",height=3D" + h ); if( win.document.body && PPTSld.g_animUseRuntime ) win.document.body.PPTSldFrameset=3Dwindow; } else { win =3D window.open( MHTMLPrefix+href,null,"fullscreen=3Dyes" ); if( win.document.body && PPTSld.g_animUseRuntime ) win.document.body.PPTSldFrameset=3Dwindow; } } function ToggleVNarration() { rObj=3DPPTSld.document.all("NSPlay") if( rObj && !PPTSld.g_animUseRuntime ) { if( (rObj.playState =3D=3D 1)||(rObj.playState =3D=3D 0) ) rObj.Play() else if( rObj.playState =3D=3D 2 ) rObj.Pause() else return; } else if( PPTSld.g_animUseRuntime ) { narObj =3D PPTSld.document.all("narrationID") if( narObj ) narObj.togglePause() } } function GetCurSldNum() { obj=3DGetHrefObj(gCurSld) if( obj.mOrigVis =3D=3D 1 ) return obj.mSldIdx else return gCurSld } function GetNumSlds() { if( GetHrefObj(gCurSld).mOrigVis =3D=3D 1 ) return GetSldList().mNumVisSlds; else return GetSldList().mList.length } function GetSldNum( href ) { for(ii=3D0; ii 1 ) PopSldList(); else if( !IsFramesMode() ) { if( gLoopCont ) GoToFirst() else EndShow() } } function GoToPrevSld() { ii=3DgCurSld-1 if( ii > 0 ) { obj=3DGetHrefObj(ii) while ( obj && ( obj.mVis =3D=3D 0 ) && ( ii>0 ) ) obj=3DGetHrefObj(--ii) if( ii =3D=3D 0 ) ii=3D1 GoToSldNum(ii) } } function GoToFirst(){ GoToSld( GetHrefObj(1).mSldHref ) } function GoToLast() { ii=3DGetSldList().mList.length if( ii !=3D gCurSld ) GoToSld( GetHrefObj(ii).mSldHref ) } function GoToSldNum( num ) { if( PPTSld.event ) PPTSld.event.cancelBubble=3Dtrue obj =3D GetHrefObj( num ) obj.mVis=3D1 gPrevSld=3DgCurSld gCurSld =3D num; PPTSld.location.replace(MHTMLPrefix+obj.mSldHref) if( IsFramesMode() ) { UpdNavPane(); UpdOtlPane(); UpdNtsPane() } } function GoToSld( href ) { if( PPTSld.event ) PPTSld.event.cancelBubble=3Dtrue GetHrefObj( GetSldNum(href) ).mVis=3D1 PPTSld.location.replace(MHTMLPrefix+href) } function SldUpdated( id ) { if( id =3D=3D GetHrefObj(gCurSld).mSldHref ) return gPrevSld=3DgCurSld gCurSld=3DGetSldNum(id) if( IsFramesMode() ) { UpdNavPane(); UpdOtlPane(); UpdNtsPane() } } function PrevSldViewed(){ GoToSld( GetHrefObj(gPrevSld).mSldHref ) } function HasPrevSld() { return ( gIsEndShow || ( gCurSld !=3D 1 && GetHrefO= bj( gCurSld-1 ).mVis =3D=3D 1 )||( GetCurSldNum() > 1 ) ) } function HasNextSld() { return (GetCurSldNum() !=3D GetNumSlds()) } function CloseWindow() { if( HideMenu() ) return; var event =3D PPTSld.event; if( !IsFramesMode() && event && (event.keyCode=3D=3D27 || event.keyCode=3D= =3D32 || event.type=3D=3D"click" ) ) window.close( self ); CatchNumKeys( self, event ); } function Unload() { gIsEndShow=3D0; } function SetupEndShow() { gIsEndShow=3D1; PPTSld.document.body.scroll=3D"no"; PPTSld.document.onkeypress=3DCloseWindow; PPTSld.document.onclick=3DCloseWindow; PPTSld.document.oncontextmenu=3D_CM; } function EndShow() { if( IsFramesMode() ) return if( PPTSld.event ) PPTSld.event.cancelBubble=3Dtrue doc=3DPPTSld.document var dir =3D doc.body.dir if( dir !=3D "rtl" ) dir =3D "ltr"; doc.open() doc.writeln('

' + ENDSHOW_MESG + '
') doc.close() } function SetSldVisited(){ GetSldList().mList[gCurSld-1].mVisited=3Dtrue } function IsSldVisited(){ return GetSldList().mList[gCurSld-1].mVisited } function hrefList( sldHref, visible, advDelay, advClk ) { this.mSldHref=3D this.mNtsHref =3D sldHref this.mOrigVis=3D this.mVis =3D visible this.mVisited=3D false this.mAdvDelay=3D advDelay this.mAdvOnClk=3D advClk } function SldList(arr,curSld,fEnd) { this.mCurSld =3D curSld; this.mList =3D new Array(); var idx =3D 1; for(ii=3D0;ii 0) { PushSldList(sldList,fEnd); gCurSld =3D 1; } else if( PPTSld.event ) PPTSld.event.cancelBubble=3Dtrue } function PushSldList(arr,fEnd) { var ii =3D gSldStack.length; gSldStack[ii] =3D new SldList(arr,gCurSld,fEnd); GoToSld( gSldStack[ii].mList[0].mSldHref ); } function PopSldList() { if (gSldStack[gSldStack.length-1].fEndShow) EndShow() else { gCurSld =3D gSldStack[gSldStack.length-1].mCurSld; gSldStack[gSldStack.length-1] =3D null; gSldStack.length--; var sldList =3D gSldStack[gSldStack.length-1]; GoToSld( sldList.mList[gCurSld - 1].mSldHref ); } } var custShowList=3Dnew Array(); function ImgBtn( oId,bId,w,action ) { var t=3Dthis t.Perform =3D _IBP t.SetActive =3D _IBSetA t.SetInactive=3D _IBSetI t.SetPressed =3D _IBSetP t.SetDisabled=3D _IBSetD t.Enabled =3D _IBSetE t.ChangeIcon =3D null t.UserAction =3D action t.ChgState =3D _IBUI t.mObjId =3D oId t.mBorderId=3D bId t.mWidth =3D w t.mIsOn =3D t.mCurState =3D 0 } function _IBSetA() { if( this.mIsOn ) { obj=3Dthis.ChgState( gHiliteClr,gShadowClr,2 ) obj.style.posTop=3D0 } } function _IBSetI() { if( this.mIsOn ) { obj=3Dthis.ChgState( gFaceClr,gFaceClr,1 ) obj.style.posTop=3D0 } } function _IBSetP() { if( this.mIsOn ) { obj=3Dthis.ChgState( gShadowClr,gHiliteClr,2 ) obj.style.posLeft+=3D1; obj.style.posTop+=3D1 } } function _IBSetD() { obj=3Dthis.ChgState( gFaceClr,gFaceClr,0 ) obj.style.posTop=3D0 } function _IBSetE( state ) { var t=3Dthis GetObj( t.mBorderId ).style.visibility=3D"visible" if( state !=3D t.mIsOn ) { t.mIsOn=3Dstate if( state ) t.SetInactive() else t.SetDisabled() } } function _IBP() { var t=3Dthis if( t.mIsOn ) { if( t.UserAction !=3D null ) t.UserAction() if( t.ChangeIcon ) { obj=3DGetObj(t.mObjId) if( t.ChangeIcon() ) obj.style.posLeft=3Dobj.style.posLeft+(t.mCurState-4)*t.mWidth else obj.style.posLeft=3Dobj.style.posLeft+(t.mCurState-0)*t.mWidth } t.SetActive() } } function _IBUI( clr1,clr2,nextState ) { var t=3Dthis SetBorder( GetObj( t.mBorderId ),clr1,clr2 ) obj=3DGetObj( t.mObjId ) obj.style.posLeft=3Dobj.style.posLeft+(t.mCurState-nextState)*t.mWidth-obj= .style.posTop t.mCurState=3DnextState return obj } function TxtBtn( oId,oeId,action,chkState ) { var t=3Dthis t.Perform =3D _TBP t.SetActive =3D _TBSetA t.SetInactive=3D _TBSetI t.SetPressed =3D _TBSetP t.SetDisabled=3D _TBSetD t.SetEnabled =3D _TBSetE t.GetState =3D chkState t.UserAction =3D action t.ChgState =3D _TBUI t.mObjId =3D oId t.m_elementsId=3D oeId t.mIsOn =3D 1 } function _TBSetA() { var t=3Dthis if( t.mIsOn && !t.GetState() ) t.ChgState( gHiliteClr,gShadowClr,0,0 ) } function _TBSetI() { var t=3Dthis if( t.mIsOn && !t.GetState() ) t.ChgState( gFaceClr,gFaceClr,0,0 ) } function _TBSetP() { if( this.mIsOn ) this.ChgState( gShadowClr,gHiliteClr,1,1 ) } function _TBSetD() { this.ChgState( gFaceClr,gFaceClr,0,0 ) this.mIsOn =3D 0 } function _TBSetE() { var t=3Dthis if( !t.GetState() ) t.ChgState( gFaceClr,gFaceClr,0,0 ) else t.ChgState( gShadowClr,gHiliteClr,1,1 ) t.mIsOn =3D 1 } function _TBP() { var t=3Dthis if( t.mIsOn ) { if( t.UserAction !=3D null ) t.UserAction() if( !t.GetState ) return if( t.GetState() ) t.SetPressed() else t.SetActive() } } function _TBUI( clr1,clr2,lOffset,tOffset ) { SetBorder( GetObj( this.mObjId ),clr1,clr2 ) Offset( GetObj( this.m_elementsId ),lOffset,tOffset ) } function Offset( obj, top, left ){ obj.style.top=3Dtop; obj.style.left=3Dle= ft } function SetBorder( obj, upperLeft, lowerRight ) { s=3Dobj.style; s.borderStyle =3D "solid" s.borderWidth =3D 1 s.borderLeftColor =3D s.borderTopColor =3D upperLeft s.borderBottomColor=3D s.borderRightColor =3D lowerRight } function GetBtnObj(){ return gBtnArr[window.event.srcElement.id] } function BtnOnOver(){ b=3DGetBtnObj(); if( b !=3D null ) b.SetActive() } function BtnOnDown(){ b=3DGetBtnObj(); if( b !=3D null ) b.SetPressed() } function BtnOnOut(){ b=3DGetBtnObj(); if( b !=3D null ) b.SetInactive() } function BtnOnUp() { b=3DGetBtnObj() if( b !=3D null ) b.Perform() else Upd() } function GetNtsState(){ return parent.gNtsOpen } function GetOtlState(){ return parent.gOtlOpen } function GetOtlTxtState(){ return parent.gOtlTxtExp } function NtsBtnSetFlag( fVal ) { s=3Ddocument.all.item( this.m_flagId ).style s.display=3D"none" if( fVal ) s.display=3D"" else s.display=3D"none" } function _BSetA_Border(){ b =3D gBtnArr[this.mObjId]; if( b !=3D null ) b.S= etActive() } function _BSetI_Border(){ b =3D gBtnArr[this.mObjId]; if( b !=3D null ) b.S= etInactive() } function _BSetP_Border(){ b =3D gBtnArr[this.mObjId]; if( b !=3D null ) b.S= etPressed() } function _BSetA_BorderImg() { b =3D gBtnArr[this.mBorderId] if( b !=3D null && this.mIsOn && !b.GetState() ) { obj=3Dthis.ChgState( gHiliteClr,gShadowClr,2 ) obj.style.posTop=3D0 } } function _BSetI_BorderImg() { b =3D gBtnArr[this.mBorderId] if( b !=3D null && this.mIsOn && !b.GetState() ) { obj=3Dthis.ChgState( gFaceClr,gFaceClr,1 ) obj.style.posTop=3D0 } } var gHiliteClr=3D"THREEDHIGHLIGHT",gShadowClr=3D"THREEDSHADOW",gFaceClr=3D"= THREEDFACE" var gBtnArr =3D new Array() gBtnArr["nb_otl"] =3D new TxtBtn( "nb_otl","nb_otlElem",parent.ToggleOtlPan= e,GetOtlState ) gBtnArr["nb_otlElem"] =3D new TxtBtn( "nb_otl","nb_otlElem",parent.ToggleOt= lPane,GetOtlState ) gBtnArr["nb_nts"] =3D new ImgBtn( "nb_nts","nb_ntsBorder",10,parent.ToggleN= tsPane ) gBtnArr["nb_nts"].SetActive =3D _BSetA_BorderImg; gBtnArr["nb_nts"].SetInactive =3D _BSetI_BorderImg; gBtnArr["nb_ntsBorder"] =3D new TxtBtn( "nb_ntsBorder","nb_ntsElem",parent.= ToggleNtsPane,GetNtsState ) gBtnArr["nb_ntsElem"] =3D new TxtBtn( "nb_ntsBorder","nb_ntsElem",parent.To= ggleNtsPane,GetNtsState ) gBtnArr["nb_prevBorder"] =3D gBtnArr["nb_prev"]=3D new ImgBtn( "nb_prev","n= b_prevBorder",30,parent.GoToPrevSld ) gBtnArr["nb_nextBorder"] =3D gBtnArr["nb_next"]=3D new ImgBtn( "nb_next","n= b_nextBorder",30,parent.GoToNextSld ) gBtnArr["nb_sldshw"]=3D new ImgBtn( "nb_sldshw","nb_sldshwBorder",18,parent= .FullScreen ) gBtnArr["nb_sldshwBorder"] =3D new TxtBtn( "nb_sldshw","nb_sldshwBorder",pa= rent.FullScreen,null ) gBtnArr["nb_sldshwBorder"].SetActive =3D _BSetA_Border; gBtnArr["nb_sldshwBorder"].SetInactive =3D _BSetI_Border; gBtnArr["nb_sldshwText"] =3D new TxtBtn( "nb_sldshw","nb_sldshwText",parent= .FullScreen,null ) gBtnArr["nb_sldshwText"].SetActive =3D _BSetA_Border; gBtnArr["nb_sldshwText"].SetInactive =3D _BSetI_Border; gBtnArr["nb_voice"] =3D gBtnArr["nb_voiceBorder"] =3D new ImgBtn( "nb_voice= ","nb_voiceBorder",18,parent.ToggleVNarration ) gBtnArr["nb_otlTxtBorder"] =3D gBtnArr["nb_otlTxt"]=3D new ImgBtn( "nb_otlT= xt","nb_otlTxtBorder",23,parent.ToggleOtlText ) gBtnArr["nb_ntsBorder"].m_flagId=3D "nb_nts" gBtnArr["nb_ntsBorder"].SetFlag =3D NtsBtnSetFlag gBtnArr["nb_otlTxt"].ChangeIcon=3D GetOtlTxtState var sNext=3D"Další",sPrev=3D"Předchozí",sEnd=3D"Kon= ec prezentace",sFont=3D"Arial",sArrow=3D"Šipka",sFreeform=3D"Voln&yacu= te; tvar",sRect=3D"Obdélník",sOval=3D"Ovál" function ShowMenu() { BuildMenu(); var doc=3DPPTSld.document.body,x=3DPPTSld.event.clientX+doc.scrollLeft,y= =3DPPTSld.event.clientY+doc.scrollTop m =3D PPTSld.document.all.item("ctxtmenu") m.style.pixelLeft=3Dx if( (x+m.scrollWidth > doc.clientWidth)&&(x-m.scrollWidth > 0) ) m.style.pixelLeft=3Dx-m.scrollWidth m.style.pixelTop=3Dy if( (y+m.scrollHeight > doc.clientHeight)&&(y-m.scrollHeight > 0) ) m.style.pixelTop=3Dy-m.scrollHeight m.style.display=3D"" } function _CM() { if( !parent.IsFullScrMode() ) return; if(!PPTSld.event.ctrlKey) { ShowMenu() return false } else HideMenu() } function BuildMenu() { if( PPTSld.document.all.item("ctxtmenu") ) return var mObj=3DCreateItem( PPTSld.document.body ) mObj.id=3D"ctxtmenu" mObj.style.visibility=3D"hidden" var s=3DmObj.style s.position=3D"absolute" s.cursor=3D"default" s.width=3D"120px" SetCMBorder(mObj,"menu","black") var iObj=3DCreateItem( mObj ) SetCMBorder( iObj, "threedhighlight","threedshadow" ) iObj.style.padding=3D2 CreateMenuItem( iObj,sNext,M_GoNextSld,M_True ) CreateMenuItem( iObj,sPrev,M_GoPrevSld,M_HasPrevSld ) CreateSeparator( iObj ) CreateMenuItem( iObj,sEnd,M_End,M_True ) mObj.style.visibility=3D"visible" } function Cancel() { window.event.cancelBubble=3Dtrue; window.event.returnVa= lue=3Dfalse } function Highlight() { ChangeClr("activecaption","threedhighlight") } function Deselect() { ChangeClr("threedface","menutext") } function Perform() { e=3DPPTSld.event.srcElement if( e.type=3D=3D"menuitem" && e.IsActive() ) e.Action() else PPTSld.event.cancelBubble=3Dtrue } function ChangeClr( bg,clr ) { e=3DPPTSld.event.srcElement if( e.type=3D=3D"menuitem" && e.IsActive() ) { e.style.backgroundColor=3Dbg e.style.color=3Dclr } } function M_HasPrevSld() { return( parent.HasPrevSld() ) } function M_GoNextSld() { if( gIsEndShow ) M_End(); 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charset="us-ascii" Molekulární genetika IV ------=_NextPart_01C945BF.EA83B520 Content-Location: file:///C:/4579C638/MolGenIV_v2008_soubory/outline.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii"

Po= známky

Prezentac= e

Osnova

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Molekulár= ní genetika IV

Ústav biologie a lékařské genetiky 1.LF UK= a VFN, Praha

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1	Molekulár= ní genetika IV Ústav biologie a lékařské genetiky 1.LF UK= a VFN, Praha
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