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Content-Location: file:///C:/94882A38/AMolGenI08_soubory/master03.xml
Content-Transfer-Encoding: quoted-printable
Content-Type: text/xml; charset="utf-8"
=
‹#›
![](3D"master03_background.gif")
BIOMOLECULES
1st semester
4th week
March 10th –
14th, 2008
![](3D"slide0016_image001.gif")
2
Task =
1,
p. 107
Carry= out replication of the following double-stranded DNA molecule:=
Carry= out replication of the following double-stranded DNA molecule:=
5´<=
/b>
T T A A C=
G C G
A T G G T C T
3´
3´<=
/b>
A A T T G=
C G C
T A C C A G A
5´
![](3D"slide0016_image002.gif")
![3D"MCj02807480000[1]"](3D"slide0016_image004.gif")
3
Replication:
Result:
![](3D"slide0020_image005.gif")
5´=
T T A A =
C G C
G A T G G T C T
3´
3´=
A A T T =
G C G
C T A C C A G A
5´
![](3D"slide0020_image006.gif")
5´=
T T A A =
C G C
G A T G G T C T
3´
3´=
A A T T =
G C G
C T A C C A G A
5´
![](3D"slide0020_image007.gif")
A T G G T=
C T
3´
T A C C A=
G A
5´
![](3D"slide0020_image009.gif")
![](3D"slide0020_image011.gif")
![](3D"slide0020_image013.gif")
![](3D"slide0020_image015.gif")
![](3D"slide0020_image016.gif")
![](3D"slide0020_image017.gif")
lagging
leading
![](3D"slide0004_image018.gif")
4
Task 2, p. 108
To the
„coding“ strand of DNA of the following sequence:
5´<=
/b>
T T A A C=
G C G
A T G G T C T
3´
form: a) „noncoding“ strand of DNA
b) mRNA
c) polypeptide
![3Dj0301060](3D"slide0004_image020.gif")
5
Solution:
![](3D"slide0005_image021.gif")
3´=
A A T T =
G C G
C T A C C A G A
5´
a) noncoding strand of DNA
![](3D"slide0005_image022.gif")
5´=
UUA =
ACG =
CGA<=
/span>
UGG<=
/span>
UCU<=
/span>
b) mRNA
c) polypeptide
Leu – Thr – Arg – Trp – Ser
![](3D"slide0006_image023.gif")
6
a)
31
A A
AUG=
met 9lys asn glu 9lys
![](3D"slide0006_image024.gif")
b)
31
U U
AUG
(met 9phe tyr val 8phe leu cys
9phe)¥
Task 3, p. 109
Carry out translation of the fictive circular =
mRNA.
![](3D"slide0007_image025.gif")
7
c)
31
C C
AUG
(met 9pro his ala 9pro cys 9 =
pro)¥
![](3D"slide0007_image026.gif")
d)
31
G <=
/span>G
AUG
(met 9gly asp 10gly trp 9gly)=
¥
8
U:C
=
2:1<=
/span>
UUU
UUC CUU UCU
CCU CUC UCC
CCC
2/3 x 2/3 x 2/3 =3D 8/27
=
2/3 x 2/3 x 1/3 =3D 4/27
=
2/3 x 1/3 x 1/3 =3D 2/27
=
1/3 x 1/3 x 1/3 =3D 1/27<=
/span>
phe =3D UUU + UUC =3D 8/27 + =
4/27 =3D
12/27
ser =3D UCU + UCC =3D 4/27 + =
2/27 =3D
6/27
leu =3D CUU + CUC =3D 4/27 + =
2/27 =3D
6/27
pro =3D CCU + CCC =3D 2/27 + =
1/27 =3D
3/27
Task 4, p. 109
9
=
Task
6, p. 110
DNA sequence:
![](3D"slide0017_image029.gif")
5´<=
/b>
A G G A T=
A T G
T T A C T C T A A A C A T
3´
a) 7 molecules of tRNA
![](3D"slide0017_image030.gif")
b) anticodons:
3´<=
b>UCC5´ 3´ UAU5´ 3´ ACA5´ 3´ AUG5´ 3´ AGA5´ 3´ UUU5´ 3´ GUA5´ =
![](3D"slide0019_image031.gif")
10
Task
7, p. 110
Consider a following tripeptide:
=
NH2 &=
#8211;
Met – Glu – Trp – COOH
DNA sequences:
![](3D"slide0019_image032.gif")
5´=
ATG<=
/span>
GAA<=
/span>
TGG<=
/span>
3´
3´=
TAC<=
/span>
CTT<=
/span>
ACC<=
/span>
![](3D"slide0019_image033.gif")
5´=
ATG<=
/span>
GAG<=
/span>
TGG<=
/span>
3´
3´=
TAC<=
/span>
CTC<=
/span>
ACC<=
/span>
DEGENERATION OF GENETIC CODE<=
/b>
![3D"eye_pract.png"](3D"slide0021_image035.gif")
11
=
In the
rat, a mutation causing cataract (eye lens opacity was identified. The
mutation is inherited in autosomal semidominant manner, homozygotes have
microphthalmia with severe lens reduction. Using linkage mapping, a DNA
segment containing the mutation was nailed down. Here you can download the mutated seq=
uence,
<=
span
style=3D'font-size:111%;font-weight:bold'>a)Differences between the sequences=
.
b)Which sequence varia=
nt can
be the mutation? (find open reading frame and compare the
polypeptides)
c)Is this finding =
relevant for human pathology? (which gene=
is mutated, what is the
function of this gene, is there an ortholog in =
human genome?)
<=
/b>
aa
Aa
AA
=
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