The Seal of First Faculty of Medicine Institute for Biology and Medical Genetics, First Faculty of Medicine, Charles University, General Teaching Hospital, Prague The Seal of Charles University

Biology and Genetics - practical exercises

Week 6 - Mitosis

Task 1

Your first task is to study the microphotograph of the mitosis. Note the size and morphological differences of the chromosomes. In order to vizualize the chromosomes - various staining - banding methods are used (in this case G-banding method).

Left-click to show chromosome:
 Chromosome 1
 Chromosome 2
 Chromosome 3
 Chromosome 4
 Chromosome. 5
 Chromosome 6
 Chromosome 7
 Chromosome 8
 Chromosome 9
 Chromosome 10
 Chromosome 11
 Chromosome 12
 Chromosome 13
 Chromosome 14
 Chromosome 15
 Chromosome 16
 Chromosome 17
 Chromosome 18
 Chromosome 19
 Chromosome 20
 Chromosome 21
 Chromosome 22
 Gonosomes
 Clear all
Mitosis 1

Task 2

Your next task is to :

  1. count all chromosomes within the mitosis (metaphase)
  2. determine the gender of the karytyped person.
Key: Count all small chromosomes of the G group (chromosomes 21, 22 and Y).
If you get 4 small chromosomes - then the chromosome Y is not present - so the karyotype is female.
But if you get 5 small chromosomes (including the Y chromosome), then the karyotype is male.
For better orientation you can see the ideograms of all human chromosomes - .

Mitóza 2

The solution will be shown in the presentation.


Task 3

Again, you have to count all chromosomes and determine the gender of the karyotyped person.

Mitóza 3

The solution will be shown in the presentation.


Task 4

Your next task is to make a karyotype - in the same way like the clinical cytogenetics in the practice. You have a little bit easier situation, because one of each chromosome pairs has already been placed. Your task is to find the second chromosome for each chromosome pair and complete the work.

Make a karyotype - Flash animation (University of Utah).